ABSTRACT
The objective of this study was to observe the infection of human cytomegalovirus (HCMV) to human umbilical vein endothelial cells, and its effect on the expression of single-stranded DNA-binding protein (SSBP1) and on lipid metabolism in endothelial cells. We screened the differential expression of mRNAs after HCMV infection by suppression subtractive hybridization and the expression levels of SSBP1 mRNA and protein after HCMV infection by real-time PCR and western blot. After verification of successful infection by indirect immunofluorescent staining and RT-PCR, we found a differential expression of lipid metabolism-related genes including LDLR, SCARB, CETP, HMGCR, ApoB and LPL induced by HCMV infection. The expression levels of SSBP1 mRNA and protein after HCMV infection were significantly down-regulated. Furthermore, we found that upregulation of SSBP1 inhibited the expression of atherosclerosis-associated LDLR, SCARB, HMGCR, CETP as well as the accumulation of lipids in the cells. The results showed that the inhibition of SSBP1 by HCMV infection promotes lipid accumulation in the cells.
Subject(s)
Humans , Cytomegalovirus Infections/metabolism , DNA-Binding Proteins/metabolism , Human Umbilical Vein Endothelial Cells/metabolism , Human Umbilical Vein Endothelial Cells/virology , Lipid Metabolism/physiology , Mitochondrial Proteins/metabolism , Atherosclerosis/metabolism , Atherosclerosis/virology , Cholesterol Ester Transfer Proteins/metabolism , Cholesterol/analysis , DNA-Binding Proteins/genetics , Down-Regulation , Hydroxymethylglutaryl CoA Reductases/metabolism , Lipid Metabolism/genetics , Mitochondrial Proteins/genetics , Receptors, LDL/metabolism , Scavenger Receptors, Class B/metabolism , Time FactorsABSTRACT
Objective To investigate the iodine nutritional level and thyroid function of pregnant and lactating women in rural areas of Jilin province. Methods The investigation sites were selected from rural areas of three towns (Baoshan, Mingcheng and Yantongshan of Panshi county, Jilin province) in 2009. The pregnant and lactating women were selected as subjects in these three towns. The blood samples were collected and the thyroid function (including serum TT3, TT4, FT3, FT4) were measured with chemiluminescence, and serum thyroglobulin antibodies(TgAb), thyromicrosome antibody(TMAb), and thyroglobulin(Tg) were measured with radioimmunoassay (RIA). The urine samples were collected three times within one month and were measured for iodine concentration by As-Ce catalytic spectrophotometry method. Results In the pregnant women, serum TT3 was higher than that of healthy pregnant women, accounted for 14.3%(8/56), while serum TT4, TT3, FT4 were lower than those of healthy pregnant women, accounted for 3.6%(2/56),5.4% (3/56), and 1.8%(1/56), respectively. In the lactating women,serum TT3 was higher than that of healthy lactating women, accounted for 3.6%(2/56), while serum TT4, FT4 were lower than those of healthy lactating women, accounted for 1.8%(1/56), respectively. Five per cent to 20% of the pregnant and lactating women had higher TgAb and TMAb. Conclusions Existing salt iodine level is appropriate for pregnant women and lactating women, but there was a tendency towards hypothyroid in some women. Routine monitoring of urinary iodine and thyroid function should be carried out among pregnant and lactating women.
ABSTRACT
Objective To evaluate the MR findings of normal and abnormal collateral ligament. Methods The MR findings of 56 normal and 36 surgically confirmed injured collateral ligaments were retrospectively reviewed and compared. Results The normal tibial and fibular collateral ligaments were hypointense string on both T 1 and T 2 coronal MR images, with an average length of 6.8 cm for tibial collateral ligament and 5.7 cm for fibular collateral ligament. 36 collateral ligaments were injured, including 18 tibial and 12 fibular collateral ligaments. Three cases (6 ligaments) had both tibial and fibularcollateral ligaments injuries. The injury of collateral ligament was classified as: Grade I (18 ligaments), Grade Ⅱ (11 ligaments), and Grade Ⅲ (7 ligaments). The complications of anterior cruciate ligament sprains (13 cases), posterior cruciate ligament sprains (9 cases), meniscal tears (12 cases), bony contusion and fracture (7 cases), and joint effusion (20 cases) were found in 25 patients(78%). Conclusion Coronal MR imaging can clearly demonstrate the structure of the knee collateral ligaments, accurately diagnose and classify the ligament injury, and correctly detect the complications. MRI should be used as a routine examining method for knee trauma.